P22 mediated recombination of frt-sites
نویسندگان
چکیده
منابع مشابه
FLP-mediated recombination of FRT sites in the maize genome.
Molecular evidence is provided for genomic recombinations in maize cells induced by the yeast FLP/FRT site-specific recombination system. The FLP protein recombined FRT sites previously integrated into the maize genome leading to excision of a selectable marker, the neo gene. NPTII activity was not observed after the successful recombination process; instead, the gusA gene was activated by the ...
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Mutants of the Sahrwneliu phage P22 have been isolated which cannot grow after infection of recombination-deficient (ret-) hosts. These mutants (designated erffor essential recombination function) are defective in a phage-specified recombination system, as shown by twoand three-factor crosses carried out in WC + and rethosts. Four erf mutants examined in detail fall into one complementation gro...
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Meiotic recombination generates gene conversion and crossover events that are distributed heterogeneously in the genome. Studies in yeast show that initiation of recombination, which occurs by the formation of DNA double-strand breaks, determines the distribution of gene conversion and crossover events that take place in nearby intervals. Recent data in humans and mice also indicate the presenc...
متن کاملThe timing of erf-mediated recombination in replication, lysogenization, and the formation of recombinant progeny by Salmonella phage P22.
Following infection, the development of phage P22 by either the lytic or the lysogenic pathways requires recombination, mediated either by the phage erf system or by the bacterial ret system [Botstein, D., and Matz, M. J. (1970) J. Mol. Biol. 54, 417-4401. We have investigated the timing of the essential recombinational processes with temperature-shift experiments using a temperature-sensitive ...
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In the absence of host RecA function, plasmid transduction by bacteriophage P22 can be mediated by Erf recombinase. Erf is not carried on the infecting particle but synthesized upon infection. In the recipient cell, Erf can promote both generalized plasmid transduction (which requires the circularization of plasmids transduced as linear multimers) and specialized plasmid transduction (which req...
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ژورنال
عنوان ژورنال: Virology
سال: 2014
ISSN: 0042-6822
DOI: 10.1016/j.virol.2014.06.015